fnctId=prof,fnctNo=195 신수임(Sooim Shin) 직위(직급) 교수 연구실 공5-241호(실험실: 단백질생화학 & 공학연구실(공5-236호)) 교수전공 및 연구분야 연락처 062-530-1848 이메일 sooim.shin@jnu.ac.kr 홈페이지 - 학력 - 박사(Ph.D.): Biochemistry, University of Mississippi Medical Center (2011) - 학사(B.S.): 응용생물화학과, 건국대학교 (2002) 경력 - 2020.8. ~ 현재: 부학장, 전남대학교 공과대학 - 2014.9.~ 현재: 부교수, 전남대학교 생물공학과 - 2011.8.~ 2014.8.: Post-Doctoral Associate, College of Medicine, University of Central Florida - 2011.10. ~ 현재: Associate Faculty, Faculty of 1000 연구 분야 - Mechanistic enzymology of biological metabolic pathways - Determine physiological effects of metal ions and protein-based free radical - Development of novel enzyme assay and biosensor using redox protein 주요연구실적 1. Sooim Shin, Moonsung Choi, Heather R.Williamson, and Victor L. Davidson. A simple method to engineer a protein-derived redox cofactor for catalysis. (2014) Biochimica et Biophysica Acta-Bioenergetics, 14, 502-507 2. Sooim Shin, Erik T. Yukl, Esha Sehanobish, Carrie M. Wilmot and Victor L. Davidson. Site-directed mutagenesis of Gln103 reveals the influence of this residue on the redox properties and stability of MauG. (2014) Biochemistry, 53(8), 1342-1349 3. Esha Sehanobish, Sooim Shin, Antonio Sanchez-Amat and Victor L. Davidson. Steady-state kinetic mechanism of LodA, a novel cysteine tryptophylquinone-dependent oxidase. (2014) FEBS Letter, 588(5), 752-756 4. Sooim Shin, and Victor L. Davidson. MauG, a diheme enzyme that catalyzes tryptophan tryptophylquinone biosynthesis by remote catalysis. (2014) Archives of Biochemistry and Biophysics, 544, 112-118 5. Sooim Shin, Manliang Feng and Victor L. Davidson, Mutation of tryptophan 93 of MauG to tyrosine causes loss of bound Ca2+andaltersthekineticmechanismoftryptophantryptophylquinonecofactorbiosynthesis.(2013)Biochemical Journal, 456(1), 129-137 6. Nafez Abu Tarboush , Erik T. Yukl, Sooim Shin, Manliang Feng, Carrie M. Wilmot, and Victor L. Davidson. Carboxyl Group of Glu113 Is Required for Stabilization of the Diferrous and Bis-FeIV States of MauG. (2013) Biochemistry, 52 (37), 6358?6367 7. Erik T. Yukl, Fange Liu, J. Krzystek, Sooim Shin, Lyndal M.R. Jensen, Victor L. Davidson, Carrie M. Wilmot and Aimin Liu. Diradical intermediate within the context of tryptophan tryptophylquinone biosynthesis. (2013) Proceedings of the National Academy of Sciences of the United States of America, 110, 4569-4573 8. Nafez Abu Tarboush, Sooim Shin, Jiafeng Geng, Aimin Liu, and Victor L. Davidson. Effects of the loss of the axial tyrosine ligand of the low-spin heme of MauG on its physical properties and reactivity. (2012) FEBS Letters, 586, 4339-4343 9. Moonsung Choi*, Sooim Shin*, and Victor L. Davidson. Characterization of electron tunneling and hole hopping reactions between different forms of MauG and methylamine dehydrogenase within a natural protein complex (2012) Biochemistry, 51, 6942-6949 *equally contribute 10. Yan Chen, Sunil G. Naik, J. Krzystek, Sooim Shin, William H. Nelson, Shenghui Xue, Jenny J. Yang, Victor L. Davidson , and Aimin Liu. Role of calcium in metalloenzymes: effects of calcium removal on the axial ligation geometry and magnetic properties of the catalytic diheme center in MauG.(2012) Biochemistry, 51, 1586-1597 11. Sooim Shin, Manglian Feng, Yan Chen, Lyndal M. R. Jensen, Hiryasu Tachikawa, Carrie M. Wilmot, Aimin Liu and Victor L. Davidson. The tightly bound calcium of MauG is required for tryptophan tryptophylquinone cofactor biosynthesis. (2011) Biochemistry, 50, 144-150 12. Sooim Shin, Nefez Abu Tarboush, and Victor L. Davdison. Long-range electron transfer reactions between hemes of MauG and different forms of tryptophan tryptophyl quinone of methylamine dehydrogenase. (2010) Biochemistry, 49, 5810-5816 13. Sooim Shin, Sheeyong Lee, and Victor L. Davidson, Suicide inactivation of MauG during reaction with O2 or H2O2 in the absence of its natural protein substrate. (2009) Biochemistry, 48, 10106-10112 14. Sheeyong Lee*, Sooim Shin*, Xianghui Li, and Victor L. Davidson. Kinetic mechanism for the initial steps in MauG-dependent tryptophan tryptophylquinone biosynthesis. (2009) Biochemistry, 48, 2442-2447. *equally contribute 15. Tae Il Jeon, Seong Gu Hwang, Nam Gyu Park, Yu Ri Jung, Sooim Shin, Sung Don Choi and Dong Ki Park. Antioxidative Effect of Chitosan on Chronic Carbon Tetrachloride Induced Hepatic Injury in Rats. (2003) Toxicology, 187, 67-73. 실험실 - 실험실명: 생화학 & 단백질공학 연구실 (Biochemistry and protein engineering Lab) - 실험실 위치 : 공5A-319 - 실험실 소개: Our research employs a broad range of biochemical, biophysical and molecular biology approaches to elucidate enzyme mechanisms, protein structure-function relationships, mechanisms of posttranslational modification of proteins, and biological energy metabolism. A general goal of this research is to describe how specific enzymes control the transfer of reactive electrons and the activation of molecular oxygen, while minimizing oxidative damage. Also it is focused on development of novel enzyme assay and biosensor using redox protein like metalloenzyme.